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dc.contributor.authorHuttunen Sanna
dc.contributor.authorToivanen Marko
dc.contributor.authorLiu Chenghai
dc.contributor.authorTikkanen-Kaukanen Carina
dc.date.accessioned2016-10-17T08:26:32Z
dc.date.available2016-10-17T08:26:32Z
dc.date.issued2016
dc.identifier10.1186/s13104-016-1838-4fi_FI
dc.identifier.citationHuttunen, S., Toivanen, M., Liu, C., & Tikkanen-Kaukanen, C. (2016). Novel anti-infective potential of salvianolic acid B against human serious pathogen Neisseria meningitidis. BMC Research Notes, 9, 25. http://doi.org/10.1186/s13104-016-1838-4fi_FI
dc.identifier.issn1756-0500
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/164
dc.descriptionArticle
dc.description.abstractBackground Epidemics of meningococcal meningitis cause significant health problems especially in Sub-Saharan Africa. Novel anti-infective candidates are needed. In modern anti-adhesion therapy initial attachment of bacteria to host cells is prevented. Our unique studies have revealed anti-adhesive candidates from natural products, namely milk and berries, against Neisseria meningitidis adhesion. In the present study against N. meningitidis adhesion, a novel binding inhibitor was found; salvianolic acid B (SA-B), a polyphenol from the radix Salviae miltiorrhizae, an important part of Chinese folk medicine. Methods In order to test inhibition of meningococcal pili binding and anti-adhesion activity of SA-B, bovine thyroglobulin, a reference glycoprotein for meningococcal receptor was used in a microtiter plate assay. Inhibitory activity was tested by using serial dilutions of SA-B extracts of 98 and 70 % purity. Results were confirmed in a HEC-1B cell dot assay and antimicrobial activity was measured by using a microbroth dilution assay. Results Almost total (93 %) inhibition of pili binding, anti-adhesion, was achieved with the 70 % extract of SA-B at the concentration of 0.3 mg/mL in the bovine thyroglobulin reference model. 50 % binding inhibition activity was achieved with 0.6 µg/mL of the SA-B extract. Total inhibition of the pili binding to HEC-1B cells was found at the tested concentration of 0.5 mg/mL. The 98 % pure SA-B resulted in weaker inhibition. At the concentration of 0.3 mg/mL 78 % inhibition was achieved in the thyroglobulin model. For 50 % inhibition 2.4 μg/mL of pure SA-B was needed. The difference between the binding inhibition activities (70 and 98 % pure SA-B) was statistically significant (P = 0.03). Antimicrobial activity of 70 % SA-B, when investigated against N. meningitidis, was detected only in relatively high concentrations. Conclusions Our results indicate that plant SA-B may prevent meningococcal infections by inhibiting meningococcal binding and may thus have an impact on the amount of nasopharyngeal carriers of N. meningitidis. This may prevent the spreading of meningococcal infections between humans. One could conclude that SA-B and its source dried radix S. miltiorrhizae, which is an important part of Chinese folk medicine, could be valuable candidates for further research in meningococcal disease prevention.fi_FI
dc.language.isoENfi_FI
dc.publisherBioMed Central Ltd.fi_FI
dc.relation.ispartofseriesBMC RESEARCH NOTES
dc.relation.urihttp://doi.org/10.1186/s13104-016-1838-4fi_FI
dc.rightsCC BY http://creativecommons.org/licenses/by/4.0/fi_FI
dc.subjectAnti-adhesion therapyfi_FI
dc.subjectAntimicrobialfi_FI
dc.subjectNeisseria meningitidisfi_FI
dc.subjectSalvianolic acidfi_FI
dc.subjectB Polyphenolfi_FI
dc.titleNovel anti-infective potential of salvianolic acid B against human serious pathogen Neisseria meningitidisfi_FI
dc.typehttp://purl.org/eprint/type/JournalArticle
dc.description.versionPublisher's pdffi_FI
dc.contributor.departmentFaculty of Health Sciences
dc.contributor.departmentSchool of Pharmacy, Activities
uef.solecris.id42043040
eprint.statushttp://purl.org/eprint/status/PeerReviewedfi_FI
dc.type.publicationinfo:eu-repo/semantics/article
dc.rights.accessrights© Authors
dc.relation.doi10.1186/s13104-016-1838-4
dc.description.reviewstatushttp://purl.org/eprint/status/PeerReviewed
dc.relation.articlenumber25
dc.relation.issue
dc.relation.volume9


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