Acetone-butanol-ethanol Fermentation from Different Pulp and Paper Manufacturing Process Side-streams
Self archived versionpublished version
MetadataShow full item record
CitationSandar, Sandra. Yang, Ming. Turunen, Ossi. Vepsäläinen, Jouko. Pappinen, Ari. Kuittinen, Suvi. (2020). Acetone-butanol-ethanol Fermentation from Different Pulp and Paper Manufacturing Process Side-streams. Bioresources, 15 (4) , 9265-9290.
The pulp and paper industry produces a diverse range of side-streams from multi-stage processes, but these remain underutilized despite their high potential for use as biofuels. This study investigated acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum DSM 1731 from the side-streams of three different stages of the pulp and paper manufacturing process (PI, PII, and PIII). Biomass specimens with and without water washing were pretreated with 0.2% H2SO4 at 180 °C for 10 min, followed by enzymatic hydrolysis, to obtain fermentable sugars. The results showed that the produced ABE solvent concentrations were 12.8 g/L, 5.2 g/L, and 6.3 g/L from PI, PII, and PIII, respectively. The butanol yields of PI, PII, and PIII were 0.25, 0.18, and 0.19 g/g sugars, respectively. Among the tested side-streams, PI was shown to have potential as a feedstock for butanol production without prewashing prior to dilute acid pretreatment, enzymatic hydrolysis, and microbial fermentation.