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dc.contributor.authorOinas J
dc.contributor.authorRieppo L
dc.contributor.authorFinnilä MAJ
dc.contributor.authorValkealahti M
dc.contributor.authorLehenkari P
dc.contributor.authorSaarakkala S
dc.date.accessioned2017-01-25T12:26:20Z
dc.date.available2017-01-25T12:26:20Z
dc.date.issued2016
dc.identifier10.1038/srep30008fi_FI
dc.identifier.issn2045-2322
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/238
dc.descriptionArticle
dc.description.abstractThe changes in chemical composition of human articular cartilage (AC) caused by osteoarthritis (OA) were investigated using Fourier transform infrared microspectroscopy (FTIR-MS). We demonstrate the sensitivity of FTIR-MS for monitoring compositional changes that occur with OA progression. Twenty-eight AC samples from tibial plateaus were imaged with FTIR-MS. Hyperspectral images of all samples were combined for K-means clustering. Partial least squares regression (PLSR) analysis was used to compare the spectra with the OARSI grade (histopathological grading of OA). Furthermore, the amide I and the carbohydrate regions were used to estimate collagen and proteoglycan contents, respectively. Spectral peak at 1338 cm−1 was used to estimate the integrity of the collagen network. The layered structure of AC was revealed using the carbohydrate region for clustering. Statistically significant correlation was observed between the OARSI grade and the collagen integrity in the superficial (r = −0.55) and the deep (r = −0.41) zones. Furthermore, PLSR models predicted the OARSI grade from the superficial (r = 0.94) and the deep (r = 0.77) regions of the AC with high accuracy. Obtained results suggest that quantitative and qualitative changes occur in the AC composition during OA progression, and these can be monitored by the use of FTIR-MS.fi_FI
dc.language.isoENfi_FI
dc.publisherSpringer Naturefi_FI
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/336267///3D-OA-HISTO
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1038/srep30008
dc.relation.ispartofseriesScientific Reports
dc.relation.urihttp://dx.doi.org/10.1038/srep30008fi_FI
dc.rightsCC BY http://creativecommons.org/licenses/by/4.0/
dc.subjectMolecular biophysicsfi_FI
dc.subjectOsteoarthritisfi_FI
dc.titleImaging of osteoarthritic Human articular cartilage using Fourier transform infrared microspectroscopy combined with multivariate and univariate analysisfi_FI
dc.typehttp://purl.org/eprint/type/JournalArticle
dc.description.versionpublisher's pdffi_FI
dc.contributor.departmentDepartment of Applied Physics, activities
uef.solecris.id42542909
eprint.statushttp://purl.org/eprint/status/PeerReviewedfi_FI
dc.type.publicationinfo:eu-repo/semantics/article
dc.rights.accessrights© Authors
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/336267///3D-OA-HISTO
dc.relation.projectidinfo:eu-repo/semantics/altIdentifier/doi/10.1038/srep30008
dc.relation.doi10.1038/srep30008
dc.description.reviewstatushttp://purl.org/eprint/status/PeerReviewed
dc.relation.articlenumber30008
dc.relation.issn2045-2322
dc.relation.issue6
dc.rights.accesslevelopenAccess


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