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dc.contributor.authorKoskela, Ali
dc.contributor.authorReinisalo, Mika
dc.contributor.authorPetrovski, Goran
dc.contributor.authorSinha, Debasish
dc.contributor.authorOlmiere, Céline
dc.contributor.authorKarjalainen, Reijo
dc.contributor.authorKaarniranta, Kai
dc.date.accessioned2017-02-14T13:26:24Z
dc.date.available2017-02-14T13:26:24Z
dc.date.issued2016
dc.identifier10.3390/nu8050284fi_FI
dc.identifier.issn2072-6643
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/272
dc.descriptionArticle
dc.description.abstractImpaired autophagic and proteasomal cleansing have been documented in aged retinal pigment epithelial (RPE) cells and age-related macular degeneration (AMD). Omega-3 fatty acids and resveratrol have many positive homeostatic effects in RPE cells. In this work, ARPE-19 cells were treated with 288 ng of Resvega, containing 30 mg of trans resveratrol and 665 mg of omega-3 fatty acids, among other nutrients, with proteasome inhibitor MG-132 or autophagy inhibitor bafilomycin A1 up to 48 h. Autophagy markers p62/SQSTM1 (p62) and LC3 (microtubule-associated protein 1A/1B-light chain 3) were analyzed by Western blotting. Fluorescence microscopy with mCherry-GFP-LC3 plasmid was applied to study the autophagy flux, and cytoprotective effects were investigated with colorimetric MTT and LDH assays. Resvega induced autophagy by showing increased autolysosome formation and autophagy flux, and the change in the p62 and LC3 protein levels further confirmed the fluorescent microscopy results. Moreover, Resvega provided a clear cytoprotection under proteasome inhibition. These findings highlight the potential of the nutraceuticals containing resveratrol, omega-3 fatty acids and other nutrients in the prevention of ARPE-19 cell damage.fi_FI
dc.language.isoENGfi_FI
dc.publisherMDPI AGfi_FI
dc.relation.ispartofseriesNutrients
dc.relation.urihttp://doi.org/10.3390/nu8050284fi_FI
dc.rightsCC BY http://creativecommons.org/licenses/by/4.0/fi_FI
dc.subjectautophagyfi_FI
dc.subjectp62/SQSTM1fi_FI
dc.subjectLC3fi_FI
dc.subjectproteasomefi_FI
dc.subjectresveratrolfi_FI
dc.subjectomega-3fi_FI
dc.titleNutraceutical with Resveratrol and Omega-3 Fatty Acids Induces Autophagy in ARPE-19 Cellsfi_FI
dc.typehttp://purl.org/eprint/type/JournalArticle
dc.description.versionpublisher's pdffi_FI
dc.contributor.departmentSchool of Medicine / Clinical Medicine
dc.contributor.departmentSchool of Pharmacy, Activities,Biologian laitos / Biologian laitoksen toiminta
uef.solecris.id41515678
eprint.statushttp://purl.org/eprint/status/PeerReviewedfi_FI
dc.type.publicationinfo:eu-repo/semantics/article
dc.rights.accessrights© Authorsfi_FI
uef.citationinfo.issue5
dc.relation.doi10.3390/nu8050284
dc.description.reviewstatushttp://purl.org/eprint/status/PeerReviewed
dc.relation.articlenumber284
dc.relation.issn2072-6643
dc.relation.issue5
dc.relation.volume8


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