Dataset Figure_1: Determination of Whi5 concentration with widefield fluorescence microscopy may be confounded by photobleaching

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Date
2022-06-04
Author(s)
Litsios, Athanasios (University of Groningen); Goswami, Pooja (Biological Sciences, Rensselaer Polytechnic Institute, Troy NY 12180, USA); Terpstra, Hanna M. (University of Groningen); Coffin, Carleton (Biological Sciences, Rensselaer Polytechnic Institute, Troy NY 12180, USA); Vuillemenot, Luc-Alban (University of Groningen); Rovetta, Mattia (University of Groningen); Ghazal, Ghada (Institute for Research in Immunology and Cancer, University of Montréal, Montréal, Quebec, Canada); Guerra, Paolo (University of Groningen); Buczak, Katarzyna (Proteomics Core Facility, Biozentrum, University of Basel, 4056 Basel, Switzerland); Schmidt, Alexander (Proteomics Core Facility, Biozentrum, University of Basel, 4056 Basel, Switzerland); Tollis, Sylvain (Institute for Research in Immunology and Cancer, University of Montréal, Montréal, Quebec, Canada and Institute of Biomedicine, University of Eastern Finland, FI-70210 Kuopio, Finland); Tyers, Mike (Institute for Research in Immunology and Cancer, University of Montréal, Montréal, Quebec, Canada); Royer, Catherine A. (Biological Sciences, Rensselaer Polytechnic Institute, Troy NY 12180, USA); Milias-Argeitis, Andreas (University of Groningen); Heinemann, Matthias (University of Groningen)
Unique identifier
https://doi.org/10.34894/SWRBYR
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Citation
Litsios, Athanasios (University of Groningen); Goswami, Pooja (Biological Sciences, Rensselaer Polytechnic Institute, Troy NY 12180, USA); Terpstra, Hanna M. (University of Groningen); Coffin, Carleton (Biological Sciences, Rensselaer Polytechnic Institute, Troy NY 12180, USA); Vuillemenot, Luc-Alban (University of Groningen); Rovetta, Mattia (University of Groningen); Ghazal, Ghada (Institute for Research in Immunology and Cancer, University of Montréal, Montréal, Quebec, Canada); Guerra, Paolo (University of Groningen); Buczak, Katarzyna (Proteomics Core Facility, Biozentrum, University of Basel, 4056 Basel, Switzerland); Schmidt, Alexander (Proteomics Core Facility, Biozentrum, University of Basel, 4056 Basel, Switzerland); Tollis, Sylvain (Institute for Research in Immunology and Cancer, University of Montréal, Montréal, Quebec, Canada and Institute of Biomedicine, University of Eastern Finland, FI-70210 Kuopio, Finland); Tyers, Mike (Institute for Research in Immunology and Cancer, University of Montréal, Montréal, Quebec, Canada); Royer, Catherine A. (Biological Sciences, Rensselaer Polytechnic Institute, Troy NY 12180, USA); Milias-Argeitis, Andreas (University of Groningen); Heinemann, Matthias (University of Groningen). , Dataset Figure_1: Determination of Whi5 concentration with widefield fluorescence microscopy may be confounded by photobleaching, 2022, https://doi.org/10.34894/SWRBYR.
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Abstract

This dataset contains all data and scripts used to generate figure 1, "Determination of Whi5 concentration with widefield fluorescence microscopy may be confounded by photobleaching".

- The ImageJ macro ("Macro_bleaching.ijm") is used to background correct the unprocessed bleaching movies,
which are stored in the folders "Bleaching GFP (movies)" and "Bleaching RFP (movies)" respectively.
- The Python notebook ("Fig1_data_analysis.ipynb") contains all code needed to analyze the bleaching movies and generate figure 1.

URI
https://erepo.uef.fi/handle/123456789/27483
Link to the original item
http://b2find.eudat.eu/dataset/8c2b0837-6c5b-42ed-a53f-e13fc34c3bee
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