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dc.contributor.authorMalinen Marjo
dc.contributor.authorNiskanen Einari A
dc.contributor.authorKaikkonen Minna U
dc.contributor.authorPalvimo Jorma J
dc.date.accessioned2017-03-02T09:44:03Z
dc.date.available2017-03-02T09:44:03Z
dc.date.issued2016
dc.identifier10.1093/nar/gkw855fi_FI
dc.identifier.issn0305-1048
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/389
dc.descriptionArticle
dc.description.abstractInflammatory processes and androgen signaling are critical for the growth of prostate cancer (PC), the most common cancer among males in Western countries. To understand the importance of potential interplay between pro-inflammatory and androgen signaling for gene regulation, we have interrogated the crosstalk between androgen receptor (AR) and NF-κB, a key transcriptional mediator of inflammatory responses, by utilizing genome-wide chromatin immunoprecipitation sequencing and global run-on sequencing in PC cells. Co-stimulation of LNCaP cells with androgen and pro-inflammatory cytokine TNFα invoked a transcriptome which was very distinct from that induced by either stimulation alone. The altered transcriptome that included gene programs linked to cell migration and invasiveness was orchestrated by significant remodeling of NF-κB and AR cistrome and enhancer landscape. Although androgen multiplied the NF-κB cistrome and TNFα restrained the AR cistrome, there was no general reciprocal tethering of the AR to the NF-κB on chromatin. Instead, redistribution of FOXA1, PIAS1 and PIAS2 contributed to the exposure of latent NF-κB chromatin-binding sites and masking of AR chromatin-binding sites. Taken together, concomitant androgen and pro-inflammatory signaling significantly remodels especially the NF-κB cistrome, reprogramming the PC cell transcriptome in fashion that may contribute to the progression of PC.fi_FI
dc.language.isoENfi_FI
dc.publisherOxford University Pressfi_FI
dc.relation.ispartofseriesNUCLEIC ACIDS RESEARCH
dc.relation.urihttp://doi.org/10.1093/nar/gkw855fi_FI
dc.rightsCC BY http://creativecommons.org/licenses/by/4.0/fi_FI
dc.subjectandrogensfi_FI
dc.subjectsignal transductionfi_FI
dc.subjectchromatinfi_FI
dc.subjectgenesfi_FI
dc.subjectandrogen receptorfi_FI
dc.subjectprostate cancerfi_FI
dc.subjectenhancer of transcriptionfi_FI
dc.titleCrosstalk between androgen and pro-inflammatory signaling remodels androgen receptor and NF-ĸB cistrome to reprogram the prostate cancer cell transcriptomefi_FI
dc.typehttp://purl.org/eprint/type/JournalArticle
dc.description.versionPublisher's pdffi_FI
dc.contributor.departmentSchool of Medicine / Biomedicine
dc.contributor.departmentA.I. Virtanen -instituutti / Bioteknologia ja molekulaarinen lääketiede
uef.solecris.id42681869
eprint.statushttp://purl.org/eprint/status/PeerReviewedfi_FI
dc.type.publicationinfo:eu-repo/semantics/article
dc.rights.accessrights© The Authorsfi_FI
uef.citationinfo.issue2
uef.citationinfo.pages619-630
dc.relation.doi10.1093/nar/gkw855
dc.description.reviewstatushttp://purl.org/eprint/status/PeerReviewed
dc.format.pagerange619-630
dc.relation.issn0305-1048
dc.relation.issue2
dc.relation.volume45


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