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MicroRNA Profiling Reveals Distinct Profiles for Tissue-Derived and Cultured Endothelial Cells

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Article (5.309Mb)
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published version
Date
2017
Author(s)
Kuosmanen Suvi M
Kansanen Emilia
Sihvola Virve
Levonen Anna-Liisa
Unique identifier
10.1038/s41598-017-11487-4
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Citation
Kuosmanen Suvi M. Kansanen Emilia. Sihvola Virve. Levonen Anna-Liisa. (2017). MicroRNA Profiling Reveals Distinct Profiles for Tissue-Derived and Cultured Endothelial Cells.  Scientific Reports, 7, 10943. 10.1038/s41598-017-11487-4.
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CC BY http://creativecommons.org/licenses/by/4.0/
Abstract

Endothelial plasticity enables the cells to switch their phenotype according to the surrounding vascular microenvironment. MicroRNAs (miRNAs) are small noncoding RNAs that control endothelial plasticity. The objective of this study was to investigate the differences in miRNA profiles of tissue-derived cells and cultured endothelial cells. To this end, miRNA expression was profiled from freshly isolated tissue-derived human vascular endothelial cells and endothelial cells cultured until cellular senescence using miRNA sequencing. In addition, the data was searched for putative novel endothelial miRNAs and miRNA isoforms. The data analysis revealed a striking change in endothelial miRNA profile as the cells adapted from tissue to cell culture environment and the overall miRNA expression decreased significantly in cultured compared to tissue-derived endothelial cells. In addition to changes in mechanosensitive miRNA expression, alterations in senescence-associated and endothelial-to-mesenchymal-transition-associated miRNAs were observed in aging cells. Collectively, the data illustrates the adaptability of endothelial cell miRNA expression that mirrors prevailing cellular environment.

Subjects
Cell biology   Non-coding RNAs   
URI
https://erepo.uef.fi/handle/123456789/5175
Link to the original item
http://dx.doi.org/10.1038/s41598-017-11487-4
Publisher
Springer Nature
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  • Terveystieteiden tiedekunta [1336]
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