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dc.contributor.authorKuosmanen Suvi M
dc.contributor.authorKansanen Emilia
dc.contributor.authorSihvola Virve
dc.contributor.authorLevonen Anna-Liisa
dc.date.accessioned2018-01-12T11:49:48Z
dc.date.available2018-01-12T11:49:48Z
dc.date.issued2017
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/5175
dc.description.abstractEndothelial plasticity enables the cells to switch their phenotype according to the surrounding vascular microenvironment. MicroRNAs (miRNAs) are small noncoding RNAs that control endothelial plasticity. The objective of this study was to investigate the differences in miRNA profiles of tissue-derived cells and cultured endothelial cells. To this end, miRNA expression was profiled from freshly isolated tissue-derived human vascular endothelial cells and endothelial cells cultured until cellular senescence using miRNA sequencing. In addition, the data was searched for putative novel endothelial miRNAs and miRNA isoforms. The data analysis revealed a striking change in endothelial miRNA profile as the cells adapted from tissue to cell culture environment and the overall miRNA expression decreased significantly in cultured compared to tissue-derived endothelial cells. In addition to changes in mechanosensitive miRNA expression, alterations in senescence-associated and endothelial-to-mesenchymal-transition-associated miRNAs were observed in aging cells. Collectively, the data illustrates the adaptability of endothelial cell miRNA expression that mirrors prevailing cellular environment.en
dc.language.isoENen
dc.publisherSpringer Natureen
dc.relation.ispartofseriesScientific Reportsen
dc.relation.urihttp://dx.doi.org/10.1038/s41598-017-11487-4en
dc.rightsCC BY http://creativecommons.org/licenses/by/4.0/en
dc.subjectCell biologyen
dc.subjectNon-coding RNAsen
dc.titleMicroRNA Profiling Reveals Distinct Profiles for Tissue-Derived and Cultured Endothelial Cellsen
dc.description.versionpublished versionen
dc.contributor.departmentA.I. Virtanen -instituuttien
uef.solecris.id49447448en
dc.type.publicationinfo:eu-repo/semantics/articleen
dc.rights.accessrights© Authorsen
dc.relation.doi10.1038/s41598-017-11487-4en
dc.description.reviewstatuspeerRevieweden
dc.relation.articlenumber10943en
dc.relation.issn2045-2322en
dc.relation.volume7en
dc.rights.accesslevelopenAccessen
dc.type.okmA1en
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
uef.solecris.openaccessOpen access -julkaisukanavassa ilmestynyt julkaisu


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