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dc.contributor.authorKoistinen, Ville
dc.contributor.authorKärnä, Riikka
dc.contributor.authorKoistinen, Arto
dc.contributor.authorArjonen, Antti
dc.contributor.authorTammi, Markku
dc.contributor.authorRilla, Kirsi
dc.date.accessioned2016-06-13T06:01:03Z
dc.date.available2016-06-13T06:01:03Z
dc.date.issued2015-10-01
dc.identifier10.1016/j.yexcr.2015.06.016
dc.identifier.citationV. Koistinen, R. Kärnä, A. Koistinen, A. Arjonen, M. Tammi, and K. Rilla, “Cell protrusions induced by hyaluronan synthase 3 (HAS3) resemble mesothelial microvilli and share cytoskeletal features of filopodia,” Experimental Cell Research, vol. 337, no. 2fi_FI
dc.identifier.issn0014-4827
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/59
dc.descriptionArticle
dc.description.abstractPrevious studies have shown that overexpression of enzymatically active GFP-HAS induces the growth of long, slender protrusions that share many features of both filopodia and microvilli. These protrusions are dependent on continuing hyaluronan synthesis, and disrupt upon digestion of hyaluronan by hyaluronidase. However, complete understanding of their nature is still missing. This work shows that the protrusions on rat peritoneal surface are ultrastructurally indistinguishable from those induced by GFP-HAS3 in MCF-7 cells. Analysis of the actin-associated proteins villin, ezrin, espin, fascin, and Myo10 indicated that the HAS3-induced protrusions share most cytoskeletal features with filopodia, but they do not require adherence to the substratum like traditional filopodia. GFP-HAS3 overexpression was found to markedly enhance filamentous actin in the protrusions and their cortical basis. Analysis of the protrusion dynamics after enzymatic digestion of hyaluronan revealed that while GFP-HAS3 escape from the protrusions and the protrusion collapse takes place immediately, the complete retraction of the protrusions occurs more slowly. This finding also suggests that hyaluronan chain maintains HAS3 in the plasma membrane. The results of this work suggest that protrusions similar to those of HAS3 overexpressing cells in vitro exist also in cells with active hyaluronan synthesis in vivo. These protrusions are similar to common filopodia but are independent of substratum attachment due to the extracellular scaffolding by the hyaluronan coat that accounts for the growth and maintenance of these structures, previously associated to invasion, adhesion and multidrug resistance.fi_FI
dc.language.isoenfi_FI
dc.publisherElsevier BVfi_FI
dc.relation.ispartofseriesExperimental Cell Research 337 (2);
dc.relation.urihttp://dx.doi.org/10.1016/j.yexcr.2015.06.016
dc.rightsCC BY-NC-ND 4.0 http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleCell protrusions induced by hyaluronan synthase 3 (HAS3) resemble mesothelial microvilli and share cytoskeletal features of filopodiafi_FI
dc.typehttp://purl.org/eprint/type/JournalArticle
dc.description.versionFinal Draft
dc.contributor.departmentFaculty of Health Sciences
uef.solecris.id36164425
eprint.statushttp://purl.org/eprint/status/PeerReviewed
dc.type.publicationinfo:eu-repo/semantics/article
dc.rights.accessrights© Elsevier Inc
dc.relation.doi10.1016/j.yexcr.2015.06.016
dc.description.reviewstatushttp://purl.org/eprint/status/PeerReviewed
dc.relation.issn0014-4827
dc.rights.accesslevelopenAccess


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