Humanin skeletal muscle protein levels increase after resistance training in men with impaired glucose metabolism

Files
Self archived version
published versionDate
2016Author(s)
Unique identifier
10.14814/phy2.13063Metadata
Show full item recordMore information
Self-archived article
Citation
Gidlund Eva-Karin. von Walden Ferdinand. Venojärvi Mika. Risérus Ulf. Heinonen Olli J. Norrbom Jessica. Sundberg Carl Johan. (2016). Humanin skeletal muscle protein levels increase after resistance training in men with impaired glucose metabolism. Physiological Reports, 4 (23) , e13063. 10.14814/phy2.13063.Rights
Licensed under
Abstract
Humanin (HN) is a mitochondrially encoded and secreted peptide linked to glucose metabolism and tissue protecting mechanisms. Whether skeletal muscle HN gene or protein expression is influenced by exercise remains unknown. In this intervention study we show, for the first time, that HN protein levels increase in human skeletal muscle following 12 weeks of resistance training in persons with prediabetes. Male subjects (n = 55) with impaired glucose regulation (IGR) were recruited and randomly assigned to resistance training, Nordic walking or a control group. The exercise interventions were performed three times per week for 12 weeks with progressively increased intensity during the intervention period. Biopsies from the vastus lateralis muscle and venous blood samples were taken before and after the intervention. Skeletal muscle and serum protein levels of HN were analyzed as well as skeletal muscle gene expression of the mitochondrially encoded gene MT‐RNR2, containing the open reading frame for HN. To elucidate mitochondrial training adaptation, mtDNA, and nuclear DNA as well as Citrate synthase were measured. Skeletal muscle HN protein levels increased by 35% after 12 weeks of resistance training. No change in humanin protein levels was seen in serum in any of the intervention groups. There was a significant correlation between humanin levels in serum and the improvements in the 2 h glucose loading test in the resistance training group. The increase in HN protein levels in skeletal muscle after regular resistance training in prediabetic males may suggest a role for HN in the regulation of glucose metabolism. Given the preventative effect of exercise on diabetes type 2, the role of HN as a mitochondrially derived peptide and an exercise‐responsive mitokine warrants further investigation.
Subjects
exercise human humanin impaired glucose regulation skeletal muscleLink to the original item
http://dx.doi.org/10.14814/phy2.13063Publisher
Wiley-BlackwellCollections
- Terveystieteiden tiedekunta [1330]