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dc.contributor.authorCai M
dc.contributor.authorBompada P
dc.contributor.authorSalehi A
dc.contributor.authorAcosta JR
dc.contributor.authorPrasad RB
dc.contributor.authorAtac D
dc.contributor.authorLaakso M
dc.contributor.authorGroop L
dc.contributor.authorDe Marinis Y
dc.date.accessioned2018-04-09T08:05:31Z
dc.date.available2018-04-09T08:05:31Z
dc.date.issued2018
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/6232
dc.description.abstractOsteopontin (OPN) is involved in various physiological processes and also implicated in multiple pathological states. It has been suggested that OPN may have a role in type 2 diabetes (T2D) by protecting pancreatic islets and interaction with incretins. However, the regulation and function of OPN in islets, especially in humans, remains largely unexplored. In this study, we performed our investigations on both diabetic mouse model SUR1-E1506K+/+ and islets from human donors. We demonstrated that OPN protein, secretion and gene expression was elevated in the diabetic SUR1-E1506K+/+ islets. We also showed that high glucose and incretins simultaneously stimulated islet OPN secretion. In islets from human cadaver donors, OPN gene expression was elevated in diabetic islets, and externally added OPN significantly increased glucose-stimulated insulin secretion (GSIS) from diabetic but not normal glycemic donors. The increase in GSIS by OPN in diabetic human islets was Ca2+ dependent, which was abolished by Ca2+-channel inhibitor isradipine. Furthermore, we also confirmed that OPN promoted cell metabolic activity when challenged by high glucose. These observations provided evidence on the protective role of OPN in pancreatic islets under diabetic condition, and may point to novel therapeutic targets for islet protection in T2D.
dc.language.isoEN
dc.publisherElsevier BV
dc.relation.ispartofseriesBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
dc.relation.urihttp://dx.doi.org/10.1016/j.bbrc.2017.11.147
dc.rightsCC BY-NC-ND https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectosteopontin
dc.subjectdiabetes
dc.subjecthyperglycemia
dc.subjectislets
dc.subjectinsulin
dc.subjectincretins
dc.titleRole of osteopontin and its regulation in pancreatic islet
dc.description.versionfinal draft
dc.contributor.departmentSchool of Medicine / Clinical Medicine
uef.solecris.id51189046en
dc.type.publicationTieteelliset aikakauslehtiartikkelit
dc.rights.accessrights© Elsevier Inc
dc.relation.doi10.1016/j.bbrc.2017.11.147
dc.description.reviewstatuspeerReviewed
dc.format.pagerange1426-1431
dc.relation.issn0006-291X
dc.relation.issue1
dc.relation.volume495
dc.rights.accesslevelopenAccess
dc.type.okmA1
uef.solecris.openaccessEi


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