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dc.contributor.authorSalo, Raimo A
dc.contributor.authorBelevich, Ilya
dc.contributor.authorManninen, Eppu
dc.contributor.authorJokitalo, Eija
dc.contributor.authorGröhn, Olli
dc.contributor.authorSierra, Alejandra
dc.date.accessioned2018-04-09T13:06:12Z
dc.date.available2018-04-09T13:06:12Z
dc.date.issued2018
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/6250
dc.description.abstractDiffusion tensor imaging (DTI) reveals microstructural features of grey and white matter non-invasively. The contrast produced by DTI, however, is not fully understood and requires further validation. We used serial block-face scanning electron microscopy (SBEM) to acquire tissue metrics, i.e., anisotropy and orientation, using three-dimensional Fourier transform-based (3D-FT) analysis, to correlate with fractional anisotropy and orientation in DTI. SBEM produces high-resolution 3D data at the mesoscopic scale with good contrast of cellular membranes. We analysed selected samples from cingulum, corpus callosum, and perilesional cortex of sham-operated and traumatic brain injury (TBI) rats. Principal orientations produced by DTI and 3D-FT in all samples were in good agreement. Anisotropy values showed similar patterns of change in corresponding DTI and 3D-FT parameters in sham-operated and TBI rats. While DTI and 3D-FT anisotropy values were similar in grey matter, 3D-FT anisotropy values were consistently lower than fractional anisotropy values from DTI in white matter. We also evaluated the effect of resolution in 3D-FT analysis. Despite small angular differences in grey matter samples, lower resolution datasets provided reliable results, allowing for analysis of larger fields of view. Overall, 3D SBEM allows for more sophisticated validation studies of diffusion imaging contrast from a tissue microstructural perspective.
dc.language.isoEN
dc.publisherElsevier BV
dc.relation.ispartofseriesNEUROIMAGE
dc.relation.urihttp://dx.doi.org/10.1016/j.neuroimage.2018.01.087
dc.rightsCC BY-NC-ND https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleQuantification of anisotropy and orientation in 3D electron microscopy and diffusion tensor imaging in injured rat brain
dc.description.versionpublished version
dc.contributor.departmentA.I. Virtanen -instituutti
uef.solecris.id52607131en
dc.type.publicationTieteelliset aikakauslehtiartikkelit
dc.rights.accessrights© Authors
dc.relation.doi10.1016/j.neuroimage.2018.01.087
dc.description.reviewstatuspeerReviewed
dc.format.pagerange404-414
dc.relation.issn1053-8119
dc.relation.volume172
dc.rights.accesslevelopenAccess
dc.type.okmA1
uef.solecris.openaccessHybridijulkaisukanavassa ilmestynyt avoin julkaisu


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