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dc.contributor.authorWang, Luning
dc.contributor.authorNissi, Mikko J
dc.contributor.authorTóth, Ferenc
dc.contributor.authorShaver, Jonah
dc.contributor.authorJohnson, Casey P
dc.contributor.authorZhang, Jinjin
dc.contributor.authorGarwood, Michael
dc.contributor.authorCarlson, Cathy S
dc.contributor.authorEllermann, Jutta M
dc.date.accessioned2016-06-13T07:25:00Z
dc.date.available2016-06-13T07:25:00Z
dc.date.issued2015-10-16
dc.identifier10.1371/journal.pone.0140400
dc.identifier.citationWang L, Nissi MJ, Tóth F, Shaver J, Johnson CP, Zhang J, et al. (2015) Multiparametric MRI of Epiphyseal Cartilage Necrosis (Osteochondrosis) with Histological Validation in a Goat Model. PLoS ONE 10(10): e0140400. doi:10.1371/journal.pone.0140400fi_FI
dc.identifier.issn1932-6203
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/62
dc.descriptionArticle
dc.description.abstractPurpose To evaluate multiple MRI parameters in a surgical model of osteochondrosis (OC) in goats. Methods Focal ischemic lesions of two different sizes were induced in the epiphyseal cartilage of the medial femoral condyles of goats at 4 days of age by surgical transection of cartilage canal blood vessels. Goats were euthanized and specimens harvested 3, 4, 5, 6, 9 and 10 weeks post-op. Ex vivo MRI scans were conducted at 9.4 Tesla for mapping the T1, T2, T1ρ, adiabatic T1ρ and TRAFF relaxation times of articular cartilage, unaffected epiphyseal cartilage, and epiphyseal cartilage within the area of the induced lesion. After MRI scans, safranin O staining was conducted to validate areas of ischemic necrosis induced in the medial femoral condyles of six goats, and to allow comparison of MRI findings with the semi-quantitative proteoglycan assessment in corresponding safranin O-stained histological sections. Results All relaxation time constants differentiated normal epiphyseal cartilage from lesions of ischemic cartilage necrosis, and the histological staining results confirmed the proteoglycan (PG) loss in the areas of ischemia. In the scanned specimens, all of the measured relaxation time constants were higher in the articular than in the normal epiphyseal cartilage, consistently allowing differentiation between these two tissues. Conclusions Multiparametric MRI provided a sensitive approach to discriminate between necrotic and viable epiphyseal cartilage and between articular and epiphyseal cartilage, which may be useful for diagnosing and monitoring OC lesions and, potentially, for assessing effectiveness of treatment interventions.fi_FI
dc.language.isoenfi_FI
dc.publisherPublic Library of Science (PLoS)fi_FI
dc.relation.ispartofseriesPLOS ONE
dc.relation.urihttp://dx.doi.org/10.1371/journal.pone.0140400
dc.rightsCC BY 4.0 http://creativecommons.org/licenses/by/4.0/
dc.subjectCartilagefi_FI
dc.subjectArticular cartilagefi_FI
dc.subjectNecrosisfi_FI
dc.subjectLesionsfi_FI
dc.subjectNMR relaxationfi_FI
dc.subjectRelaxation timefi_FI
dc.subjectGoatsfi_FI
dc.subjectMagnetic resonance imagingfi_FI
dc.titleMultiparametric MRI of Epiphyseal Cartilage Necrosis (Osteochondrosis) with Histological Validation in a Goat Modelfi_FI
dc.typehttp://purl.org/eprint/type/JournalArticle
dc.description.versionPublisher's pdf
dc.contributor.departmentFaculty of Science and Forestry
uef.solecris.id37368250
eprint.statushttp://purl.org/eprint/status/PeerReviewed
dc.type.publicationinfo:eu-repo/semantics/article
dc.rights.accessrights© Authors
dc.relation.doi10.1371/journal.pone.0140400
dc.description.reviewstatushttp://purl.org/eprint/status/PeerReviewed
dc.relation.issn1932-6203
dc.relation.issue10
dc.relation.volume10
dc.rights.accesslevelopenAccess


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