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dc.contributor.authorStefanutti, E
dc.contributor.authorSierra, A
dc.contributor.authorMiocchi, P
dc.contributor.authorMassimi, L
dc.contributor.authorBrun, F
dc.contributor.authorMaugeri, L
dc.contributor.authorBukreeva, I
dc.contributor.authorNurmi, A
dc.contributor.authorProvinciali, G Begani
dc.contributor.authorTromba, G
dc.contributor.authorGrohn, O
dc.contributor.authorGiove, F
dc.contributor.authorCedola, A
dc.contributor.authorFratini, M
dc.date.accessioned2018-04-13T07:07:10Z
dc.date.available2018-04-13T07:07:10Z
dc.date.issued2018
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/6314
dc.description.abstractSynchrotron X-ray Phase Contrast micro-Tomography (SXrPCμT) is a powerful tool in the investigation of biological tissues, including the central nervous system (CNS), and it allows to simultaneously detect the vascular and neuronal network avoiding contrast agents or destructive sample preparations. However, specific sample preparation procedures aimed to optimize the achievable contrast- and signal-to-noise ratio (CNR and SNR, respectively) are required. Here we report and discuss the effects of perfusion with two different fixative agents (ethanol and paraformaldehyde) and with a widely used contrast medium (MICROFIL®) on mouse spinal cord. As a main result, we found that ethanol enhances contrast at the grey/white matter interface and increases the contrast in correspondence of vascular features and fibres, thus providing an adequate spatial resolution to visualise the vascular network at the microscale. On the other hand, ethanol is known to induce tissue dehydration, likely reducing cell dimensions below the spatial resolution limit imposed by the experimental technique. Nonetheless, neurons remain well visible using either perfused paraformaldehyde or MICROFIL® compound, as these latter media do not affect tissues with dehydration effects. Paraformaldehyde appears as the best compromise: it is not a contrast agent, like MICROFIL®, but it is less invasive than ethanol and permits to visualise well both cells and blood vessels. However, a quantitative estimation of the relative grey matter volume of each sample has led us to conclude that no significant alterations in the grey matter extension compared to the white matter occur as a consequence of the perfusion procedures tested in this study.
dc.language.isoEN
dc.publisherIOP Publishing
dc.relation.ispartofseriesJournal of Instrumentation
dc.relation.urihttp://dx.doi.org/10.1088/1748-0221/13/03/C03027
dc.rightsCC BY http://creativecommons.org/licenses/by/4.0/
dc.subjectmedical-image reconstruction methods and algorithms
dc.subjectcomputer-aided diagnosis
dc.subjectmedical-image reconstruction methods and algorithms
dc.subjectcomputer-aided software
dc.titleAssessment of the effects of different sample perfusion procedures on phase-contrast tomographic images of mouse spinal cord
dc.description.versionpublished version
dc.contributor.departmentA.I. Virtanen -instituutti
uef.solecris.id53643656en
dc.type.publicationTieteelliset aikakauslehtiartikkelit
dc.rights.accessrights© Authors
dc.relation.doi10.1088/1748-0221/13/03/C03027
dc.description.reviewstatuspeerReviewed
dc.relation.articlenumberC03027
dc.relation.issn1748-0221
dc.relation.volume13
dc.rights.accesslevelopenAccess
dc.type.okmA1
uef.solecris.openaccessOpen access -julkaisukanavassa ilmestynyt julkaisu


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