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dc.contributor.authorAvdic, U
dc.contributor.authorAhl, M
dc.contributor.authorChugh, D
dc.contributor.authorAli, I
dc.contributor.authorChary, K
dc.contributor.authorSierra, A
dc.contributor.authorEkdahl, CT
dc.date.accessioned2018-04-17T07:28:12Z
dc.date.available2018-04-17T07:28:12Z
dc.date.issued2018
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/6521
dc.description.abstractObjective Status epilepticus (SE) is an abnormally prolonged epileptic seizure that if associated with convulsive motor symptoms is potentially life threatening for a patient. However, 20%‐40% of patients with SE lack convulsive events and instead present with more subtle semiology such as altered consciousness and less motor activity. Today, there is no general consensus regarding to what extent nonconvulsive SE (NCSE) is harmful to the brain, which adds uncertainty to stringent treatment regimes. Methods Here, we evaluated brain pathology in an experimental rat and mouse model of complex partial NCSE originating in the temporal lobes with Western blot analysis, immunohistochemistry, and ex vivo diffusion tensor imaging (DTI). The NCSE was induced by electrical stimulation with intrahippocampal electrodes and terminated with pentobarbital anesthesia. Video‐electroencephalographic recordings were performed throughout the experiment. Results DTI of mice 7 weeks post‐NCSE showed no robust long‐lasting changes in fractional anisotropy within the hippocampal epileptic focus. Instead, we found pathophysiological changes developing over time when measuring protein levels and cell counts in extracted brain tissue. At 6 and 24 hours post‐NCSE in rats, few changes were observed within the hippocampus and cortical or subcortical structures in Western blot analyses of key components of the cellular immune response and synaptic protein expression, while neurodegeneration had started. However, 1 week post‐NCSE, both excitatory and inhibitory synaptic protein levels were decreased in hippocampus, concomitant with an excessive microglial and astrocytic activation. At 4 weeks, a continuous immune response in the hippocampus was accompanied with neuronal loss. Levels of the excitatory synaptic adhesion molecule N‐cadherin were decreased specifically in rats that developed unprovoked spontaneous seizures (epileptogenesis) within 1 month following NCSE, compared to rats only exhibiting acute symptomatic seizures within 1 week post‐NCSE. Significance These findings provide evidence for a significant brain pathology following NCSE in an experimental rodent model.
dc.language.isoEN
dc.publisherWiley
dc.relation.ispartofseriesEPILEPSIA (NY)
dc.relation.urihttp://dx.doi.org/10.1111/epi.14070
dc.rightsCC BY-NC http://creativecommons.org/licenses/by-nc/4.0/
dc.subjectdiffusion tensor imaging
dc.subjectinflammation
dc.subjectN-cadherin
dc.subjectnonconvulsive status epilepticus
dc.subjectsynaptic proteins
dc.titleNonconvulsive status epilepticus in rats leads to brain pathology
dc.description.versionpublished version
dc.contributor.departmentA.I. Virtanen -instituutti
dc.contributor.departmentA.I. Virtanen -instituutti
uef.solecris.id53847278en
dc.type.publicationTieteelliset aikakauslehtiartikkelit
dc.rights.accessrights© Authors
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/ FP7-HEALTH/602102/EU/Targets and biomarkers for antiepileptogenesis/EPITARGET
dc.relation.doi10.1111/epi.14070
dc.description.reviewstatuspeerReviewed
dc.format.pagerange945-958
dc.relation.issn0013-9580
dc.relation.issue5
dc.relation.volume59
dc.rights.accesslevelopenAccess
dc.type.okmA1
uef.solecris.openaccessHybridijulkaisukanavassa ilmestynyt avoin julkaisu


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