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dc.contributor.authorWiniarczyk, M
dc.contributor.authorKaarniranta, K
dc.contributor.authorWiniarczyk, S
dc.contributor.authorAdaszek, ¿
dc.contributor.authorWiniarczyk, D
dc.contributor.authorMackiewicz, J
dc.date.accessioned2018-05-04T08:42:45Z
dc.date.available2018-05-04T08:42:45Z
dc.date.issued2018
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/6569
dc.description.abstractPurpose Age-related macular degeneration (AMD) is the main reason for blindness in elderly people in the developed countries. Current screening protocols have limitations in detecting the early signs of retinal degeneration. Therefore, it would be desirable to find novel biomarkers for early detection of AMD. Development of novel biomarkers would help in the prevention, diagnostics, and treatment of AMD. Proteomic analysis of tear film has shown promise in this research area. If an optimal set of biomarkers could be obtained from accessible body fluids, it would represent a reliable way to monitor disease progression and response to novel therapies. Methods Tear films were collected on Schirmer strips from a total of 22 patients (8 with wet AMD, 6 with dry AMD, and 8 control individuals). 2D electrophoresis was used to separate tear film proteins prior to their identification with matrix-assisted laser desorption/ionization time of flight spectrometer (MALDI-TOF/TOF) and matching with functional databases. Results A total of 342 proteins were identified. Most of them were previously described in various proteomic studies concerning AMD. Shootin-1, histatin-3, fidgetin-like protein 1, SRC kinase signaling inhibitor, Graves disease carrier protein, actin cytoplasmic 1, prolactin-inducible protein 1, and protein S100-A7A were upregulated in the tear film samples isolated from AMD patients and were not previously linked with this disease in any proteomic analysis. Conclusion The upregulated proteins supplement our current knowledge of AMD pathogenesis, providing evidence that certain specific proteins are expressed into the tear film in AMD. As far we are aware, this is the first study to have undertaken a comprehensive in-depth analysis of the human tear film proteome in AMD patients.
dc.language.isoEN
dc.publisherSpringer Nature
dc.relation.ispartofseriesGRAEFE'S ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
dc.relation.urihttp://dx.doi.org/10.1007/s00417-018-3984-y
dc.rightsCC BY http://creativecommons.org/licenses/by/4.0/
dc.subjectage-related macular degeneration
dc.subjectbiomarker
dc.subjectproteomics
dc.subjecttear film
dc.titleTear film proteome in age-related macular degeneration
dc.description.versionpublished version
dc.contributor.departmentSchool of Medicine / Clinical Medicine
uef.solecris.id54192519en
dc.type.publicationTieteelliset aikakauslehtiartikkelit
dc.rights.accessrights© Authors
dc.relation.doi10.1007/s00417-018-3984-y
dc.description.reviewstatuspeerReviewed
dc.relation.issn0721-832X
dc.relation.volumeFirst Online: 25 April 2018
dc.rights.accesslevelopenAccess
dc.type.okmA1
uef.solecris.openaccessHybridijulkaisukanavassa ilmestynyt avoin julkaisu


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