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dc.contributor.authorOjanen, SP
dc.contributor.authorFinnilä, MAJ
dc.contributor.authorReunamo, AE
dc.contributor.authorRonkainen, AP
dc.contributor.authorMikkonen, S
dc.contributor.authorHerzog, W
dc.contributor.authorSaarakkala, S
dc.contributor.authorKorhonen, RK
dc.date.accessioned2018-05-04T09:50:57Z
dc.date.available2018-05-04T09:50:57Z
dc.date.issued2018
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/6571
dc.description.abstractIntroduction One of the characteristics of early osteoarthritis (OA) is the loss of fixed charged density (FCD) of glycosaminoglycans in the superficial zone of articular cartilage. However, possible local changes in the FCD content of the pericellular matrix (PCM) are not fully understood. Hence, our aim was to investigate the effect of unilateral anterior cruciate ligament transection (ACLT) in rabbit knees on estimated FCD in the PCM compared to that in the ECM, and relate these results with cell morphology. Methods Articular cartilage samples were collected from ACLT, contralateral and intact control knee joints from lateral and medial femoral condyles and tibial plateaus, and from the femoral groove and patella. Histological samples were prepared and stained with Safranin-O to estimate the FCD content around the chondrocytes in the PCM and the ECM with digital densitometry. Results As a result of ACLT, the greatest decreases in the FCD content of the PCM were observed in the superficial zone of the lateral femoral condyle (p = 0.02), medial tibial plateau (p = 0.002) and patellar (p < 0.001) cartilage. The normalized FCD content of the PCM compared to the surrounding ECM was increased most in the femoral condyles (p < 0.01) and medial tibial plateau (p = 0.02) cartilage. The high normalized FCD content of the PCM in the superficial zone of lateral femoral condyle cartilage was consistent with the round cell morphology in that location. Conclusions In conclusion, we suggest that certain sites in the knee joint, particularly the lateral femoral condyle cartilage, experience less FCD loss in the PCM than in the ECM in early post-traumatic OA, which could lead to altered cell shape.
dc.language.isoEN
dc.publisherPublic Library of Science (PLoS)
dc.relation.ispartofseriesPLOS ONE
dc.relation.urihttp://dx.doi.org/10.1371/journal.pone.0196203
dc.rightsCC BY http://creativecommons.org/licenses/by/4.0/
dc.titleSite-specific glycosaminoglycan content is better maintained in the pericellular matrix than the extracellular matrix in early post-traumatic osteoarthritis
dc.description.versionpublished version
dc.contributor.departmentDepartment of Applied Physics, activities
uef.solecris.id54192568en
dc.type.publicationTieteelliset aikakauslehtiartikkelit
dc.rights.accessrights© Authors
dc.relation.doi10.1371/journal.pone.0196203
dc.description.reviewstatuspeerReviewed
dc.relation.articlenumbere0196203
dc.relation.issn1932-6203
dc.relation.issue4
dc.relation.volume13
dc.rights.accesslevelopenAccess
dc.type.okmA1
uef.solecris.openaccessOpen access -julkaisukanavassa ilmestynyt julkaisu


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