Consensus guidelines for the use and interpretation of angiogenesis assays
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CitationNowak-Sliwinska, P. Alitalo, K. Allen, E. Anisimov, A. Aplin, AC. Auerbach, R. Augustin, HG. Bates, DO. van Beijnum, JR. Bender, RHF. Bergers, G. Bikfalvi, A. Bischoff, J. Böck, BC. Brooks, PC. Bussolino, F. Cakir, B. Carmeliet, P. Castranova, D. Cimpean, AM. et al [Incl Yläherttuala, Seppo; Nurro, Jussi]. (2018). Consensus guidelines for the use and interpretation of angiogenesis assays. Angiogenesis, First Online: 15 May 2018, 10.1007/s10456-018-9613-x.
The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference.
Subjectsangiogenesis aortic ring endothelial cell migration proliferation microfluidic zebrafish chorioallantoic membrane (CAM) vascular network intussusceptive angiogenesis retinal vasculature corneal angiogenesis hindlimb ischemia myocardial angiogenesis recombinant proteins tip cells plug assay myocardial angiogenesis vessel co-option
Link to the original itemhttp://dx.doi.org/10.1007/s10456-018-9613-x
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