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dc.contributor.authorKhamidakh,Amna E Abu
dc.contributor.authorRodriguez-Martinez, Alejandra
dc.contributor.authorKaarniranta, Kai
dc.contributor.authorKallioniemi, Anne
dc.contributor.authorSkottman, Heli
dc.contributor.authorHyttinen, Jari
dc.contributor.authorJuuti-Uusitalo, Kati
dc.date.accessioned2018-08-24T11:28:16Z
dc.date.available2018-08-24T11:28:16Z
dc.date.issued2018
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/6853
dc.description.abstractBackground Wound healing of retinal pigment epithelium (RPE) is a complex process that may take place in common age-related macular degeneration eye disease. The purpose of this study was to evaluate whether wounding and wound healing has an effect on Ca2+ dynamics in human embryonic stem cell (hESC)-RPEs cultured different periods of time. Methods The 9-day-cultured or 28-day-cultured hESC-RPEs from two different cell lines were wounded and the dynamics of spontaneous and mechanically induced intracellular Ca2+ activity was measured with live-cell Ca2+ imaging either immediately or 7 days after wounding. The healing time and speed were analyzed with time-lapse bright field microscopy. The Ca2+ activity and healing speed were analysed with image analysis. In addition the extracellular matrix deposition was assessed with confocal microscopy. Results The Ca2+ dynamics in hESC-RPE monolayers differed depending on the culture time: 9-day-cultured cells had higher number of cells with spontaneous Ca2+ activity close to freshly wounded edge compared to control areas, whereas in 28-day-cultured cells there was no difference in wounded and control areas. The 28-day-cultured, wounded and 7-day-healed hESC-RPEs produced wide-spreading intercellular Ca2+ waves upon mechanical stimulation, while in controls propagation was restricted. Most importantly, both wave spreading and spontaneous Ca2+ activity of cells within the healed area, as well as the cell morphology of 28-day-cultured, wounded and thereafter 7-day-healed areas resembled the 9-day-cultured hESC-RPEs. Conclusions This acquired knowledge about Ca2+ dynamics of wounded hESC-RPE monolayers is important for understanding the dynamics of RPE wound healing, and could offer a reliable functionality test for RPE cells. The data presented in here suggests that assessment of Ca2+ dynamics analysed with image analysis could be used as a reliable non-invasive functionality test for RPE cells.
dc.language.isoenglanti
dc.publisherSpringer Nature America, Inc
dc.relation.ispartofseriesBiomedical engineering online
dc.relation.urihttp://dx.doi.org/10.1186/s12938-018-0535-z
dc.rightsCC BY http://creativecommons.org/licenses/by/4.0/
dc.subjectimage analysis
dc.subjecthESC-RPE
dc.subjectRPE
dc.subjectcell maturation
dc.subjectwound healing
dc.subjectspontaneous [Ca2+]i increases
dc.subjectmechanical stimulation
dc.subjectCa2+ waves
dc.subjectmechanically induced intercellular Ca2+ waves
dc.titleWound healing of human embryonic stem cell-derived retinal pigment epithelial cells is affected by maturation stage
dc.description.versionpublished version
dc.contributor.departmentSchool of Medicine / Clinical Medicine
uef.solecris.id56540527en
dc.type.publicationTieteelliset aikakauslehtiartikkelit
dc.rights.accessrights© Authors
dc.relation.doi10.1186/s12938-018-0535-z
dc.description.reviewstatuspeerReviewed
dc.relation.articlenumber102
dc.relation.volume17
dc.rights.accesslevelopenAccess
dc.type.okmA1
uef.solecris.openaccessOpen access -julkaisukanavassa ilmestynyt julkaisu


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