Skip to main contentSkip to search and navigation

UEF eREPOSITORY

    • English
    • suomi
  • English 
    • English
    • suomi
  • Login
View Item 
  •   Home
  • Artikkelit
  • Terveystieteiden tiedekunta
  • View Item
  •   Home
  • Artikkelit
  • Terveystieteiden tiedekunta
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Nucleic acid delivery to differentiated retinal pigment epithelial cells using cell-penetrating peptide as a carrier

Thumbnail
Files
Article (1.434Mb)
Self archived version
final draft
Date
2019
Author(s)
Subia, B
Reinisalo, M
Dey, N
Tavakoli, S
Subrizi, A
Ganguli, M
Ruponen, M
Unique identifier
10.1016/j.ejpb.2019.05.003
Metadata
Show full item record
More information
Research Database SoleCris

Self-archived article

Citation
Subia, B. Reinisalo, M. Dey, N. Tavakoli, S. Subrizi, A. Ganguli, M. Ruponen, M. (2019). Nucleic acid delivery to differentiated retinal pigment epithelial cells using cell-penetrating peptide as a carrier.  European journal of pharmaceutics and biopharmaceutics, 140, 91-99. 10.1016/j.ejpb.2019.05.003.
Rights
© Elsevier B.V.
Licensed under
CC BY-NC-ND https://creativecommons.org/licenses/by-nc-nd/4.0/
Abstract

Nucleic acid delivery to the eye is a promising treatment strategy for many retinal disorders. In this manuscript, retinal gene delivery with non-coated and chondroitin sulphate (CS) coated amphipathic and cationic peptides was tested. The transfection and gene knockdown efficiencies were evaluated in different retinal pigment epithelial (RPE) cell models including both dividing and differentiated cells. In addition, the mobility of peptide-based gene delivery systems was examined in porcine vitreous by particle tracking analysis. The results indicate that amphipathic and cationic peptides are safe in vitro and are capable of high transgene expression and gene knockdown in dividing cells. We further demonstrate that incorporation of CS improves the efficiency of gene delivery of peptide-based systems. Most importantly, the transgene expression mediated by both non-coated and CS coated peptides was high in differentiated as well as in human primary RPE cells which are typically difficult to transfect. Coating of peptide-based gene delivery systems with CS improved diffusion in the vitreous and enhanced the stability of the polyplexes. The results indicate that a peptide-based system can be fine-tuned as a promising approach for retinal gene delivery.

Subjects
polyplexe   peptide carrier   chondroitin sulphate   retinal cell line   gene delivery   
URI
https://erepo.uef.fi/handle/123456789/7645
Link to the original item
http://dx.doi.org/10.1016/j.ejpb.2019.05.003
Publisher
Elsevier BV
Collections
  • Terveystieteiden tiedekunta [1337]
University of Eastern Finland
OpenAccess
eRepo
erepo@uef.fi
UEF Open Science
Accessibility in eRepo
Service provided by
the University of Eastern Finland Library
Library web pages
Twitter
Facebook
Youtube
Library blog
 sitemap
Search

Browse

All of the ArchiveResource types & CollectionsBy Issue DateAuthorsTitlesSubjectsFacultyDepartmentFull organizationSeriesMain subjectThis CollectionBy Issue DateAuthorsTitlesSubjectsFacultyDepartmentFull organizationSeriesMain subject

My Account

Login
University of Eastern Finland
OpenAccess
eRepo
erepo@uef.fi
UEF Open Science
Accessibility in eRepo
Service provided by
the University of Eastern Finland Library
Library web pages
Twitter
Facebook
Youtube
Library blog
 sitemap