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dc.contributor.authorHassinen, M
dc.contributor.authorKorajoki, H
dc.contributor.authorAbramochkin, D
dc.contributor.authorKrivosheya, P
dc.contributor.authorVornanen, M
dc.date.accessioned2019-12-13T12:53:10Z
dc.date.available2019-12-13T12:53:10Z
dc.date.issued2019
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/7872
dc.description.abstractInward rectifier K+ (Kir2) channels are critical for electrical excitability of cardiac myocytes. Here, we examine expression of Kir2 channels in the heart of three Gadiformes species, polar cod (Boreogadus saida) and navaga (Eleginus nawaga) of the Arctic Ocean and burbot (Lota lota) of the temperate lakes to find out the role of Kir2 channels in cardiac adaptation to cold. Five boreal freshwater species: brown trout (Salmo trutta fario), arctic char (Salvelinus alpinus), roach (Rutilus rutilus), perch (Perca fluviatilis) and pike (Esox lucius), and zebrafish (Danio rerio), were included for comparison. Transcript expression of genes encoding Kir2.1a, − 2.1b, − 2.2a, − 2.2b and − 2.4 was studied from atrium and ventricle of thermally acclimated or acclimatized fish by quantitative PCR. Kir2 composition in the polar cod was more diverse than in other species in that all Kir2 isoforms were relatively highly expressed. Kir2 composition of navaga and burbot differed from that of the polar cod as well as from those of other species. The relative expression of Kir2.2 transcripts, especially Kir2.2b, was higher in both atrium and ventricle of navaga and burbot (56–89% from the total Kir2 pool) than in other species (0.1–11%). Thermal acclimation induced only small changes in cardiac Kir2 transcript expression in Gadiformes species. However, Kir2.2b transcripts were upregulated in cold-acclimated navaga and burbot hearts. All in all, the cardiac Kir2 composition seems to be dependent on both phylogenetic position and thermal preference of the fish.
dc.language.isoenglanti
dc.relation.ispartofseriesJournal of comparative physiology B : biochemical, systemic, and environmental physiology
dc.relation.urihttp://dx.doi.org/10.1007/s00360-019-01241-9
dc.rightsCC BY http://creativecommons.org/licenses/by/4.0/
dc.subjectfish heart
dc.subjectinward rectifier potassium channels
dc.subjectKir2 paralogues
dc.subjectthermal acclimation
dc.subjectRT-qPCR
dc.titleTranscript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species
dc.description.versionfinal draft
dc.contributor.departmentYmpäristö- ja biotieteiden laitos / Toiminta
uef.solecris.id66361499en
dc.type.publicationTieteelliset aikakauslehtiartikkelit
dc.rights.accessrights© Authors
dc.relation.doi10.1007/s00360-019-01241-9
dc.description.reviewstatuspeerReviewed
dc.format.pagerange735-749
dc.publisher.countrySaksa
dc.relation.issn0174-1578
dc.relation.issue6
dc.relation.volume189
dc.rights.accesslevelopenAccess
dc.type.okmA1
uef.solecris.openaccessHybridijulkaisukanavassa ilmestynyt avoin julkaisu


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