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dc.contributor.authorHonkanen, MKM
dc.contributor.authorSaukko, AEA
dc.contributor.authorTurunen, MJ
dc.contributor.authorShaikh, R
dc.contributor.authorPrakash, M
dc.contributor.authorLovric, G
dc.contributor.authorJoukainen, A
dc.contributor.authorKröger, H
dc.contributor.authorGrinstaff, MW
dc.contributor.authorTöyräs, J
dc.date.accessioned2020-01-23T08:21:46Z
dc.date.available2020-01-23T08:21:46Z
dc.date.issued2020
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/7967
dc.description.abstractDual contrast micro computed tomography (CT) shows potential for detecting articular cartilage degeneration. However, the performance of conventional CT systems is limited by beam hardening, low image resolution (full‐body CT), and long acquisition times (conventional microCT). Therefore, to reveal the full potential of the dual contrast technique for imaging cartilage composition we employ the technique using synchrotron microCT. We hypothesize that the above‐mentioned limitations are overcome with synchrotron microCT utilizing monochromatic X‐ray beam and fast image acquisition. Human osteochondral samples (n = 41, four cadavers) were immersed in a contrast agent solution containing two agents (cationic CA4+ and non‐ionic gadoteridol) and imaged with synchrotron microCT at an early diffusion time point (2 h) and at diffusion equilibrium (72 h) using two monochromatic X‐ray energies (32 and 34 keV). The dual contrast technique enabled simultaneous determination of CA4+ (i.e., proteoglycan content) and gadoteridol (i.e., water content) partitions within cartilage. Cartilage proteoglycan content and biomechanical properties correlated significantly (0.327 < r < 0.736, p < 0.05) with CA4+ partition in superficial and middle zones at both diffusion time points. Normalization of the CA4+ partition with gadoteridol partition within the cartilage significantly (p < 0.05) improved the detection sensitivity for human osteoarthritic cartilage proteoglycan content, biomechanical properties, and overall condition (Mankin, Osteoarthritis Research Society International, and International Cartilage Repair Society grading systems). The dual energy technique combined with the dual contrast agent enables assessment of human articular cartilage proteoglycan content and biomechanical properties based on CA4+ partition determined using synchrotron microCT. Additionally, the dual contrast technique is not limited by the beam hardening artifact of conventional CT systems.
dc.language.isoenglanti
dc.publisherWiley
dc.relation.ispartofseriesJournal of orthopaedic research
dc.relation.urihttp://dx.doi.org/10.1002/jor.24479
dc.rightsCC BY http://creativecommons.org/licenses/by/4.0/
dc.subjectdual contrast agent
dc.subjectCA4+
dc.subjectcationic contrast agent
dc.subjectcontrast‐enhanced computed tomography
dc.subjectCECT
dc.titleSynchrotron microCT reveals the potential of the dual contrast technique for quantitative assessment of human articular cartilage composition
dc.description.versionpublished version
dc.contributor.departmentDepartment of Applied Physics, activities
dc.contributor.departmentSchool of Medicine / Clinical Medicine,A.I. Virtanen -instituutti
uef.solecris.id65332966en
dc.type.publicationTieteelliset aikakauslehtiartikkelit
dc.rights.accessrights© Authors
dc.relation.doi10.1002/jor.24479
dc.description.reviewstatuspeerReviewed
dc.format.pagerange563-573
dc.relation.issn0736-0266
dc.relation.issue3
dc.relation.volume38
dc.rights.accesslevelopenAccess
dc.type.okmA1
uef.solecris.openaccessEi


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