dc.contributor.author | Bhattarai, Niina | |
dc.contributor.author | Korhonen, Eveliina | |
dc.contributor.author | Toppila, Maija | |
dc.contributor.author | Koskela, Ali | |
dc.contributor.author | Kaarniranta, Kai | |
dc.contributor.author | Mysore, Yashavanthi | |
dc.contributor.author | Kauppinen, Anu | |
dc.date.accessioned | 2020-04-02T11:11:01Z | |
dc.date.available | 2020-04-02T11:11:01Z | |
dc.date.issued | 2020 | |
dc.identifier.uri | https://erepo.uef.fi/handle/123456789/8080 | |
dc.description.abstract | Retinal pigment epithelial (RPE) cells maintain homeostasis at the retina and they are under continuous oxidative stress. Cigarette smoke is a prominent environmental risk factor for age-related macular degeneration (AMD), which further increases the oxidant load in retinal tissues. In this study, we measured oxidative stress and inflammatory markers upon cigarette smoke-derived hydroquinone exposure on human ARPE-19 cells. In addition, we studied the effects of commercial Resvega product on hydroquinone-induced oxidative stress. Previously, it was observed that Resvega induces autophagy during impaired protein clearance in ARPE-19 cells, for which it has the potential to alleviate pro-inflammatory pathways. Cell viability was determined while using the lactate dehydrogenase (LDH) and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, and the cytokine levels were measured using the enzyme-linked immunosorbent assay (ELISA). Reactive oxygen species (ROS) production were measured using the 2′,7′-dichlorofluorescin diacetate (H2DCFDA) probe. Hydroquinone compromised the cell viability and increased ROS production in ARPE-19 cells. Resvega significantly improved cell viability upon hydroquinone exposure and reduced the release of interleukin (IL)-8 and monocytic chemoattractant protein (MCP)-1 from RPE cells. Resvega, N-acetyl-cysteine (NAC) and aminopyrrolidine-2,4-dicarboxylic acid (APDC) alleviated hydroquinone-induced ROS production in RPE cells. Collectively, our results indicate that hydroquinone induces cytotoxicity and increases oxidative stress through NADPH oxidase activity in RPE cells, and resveratrol-containing Resvega products prevent those adverse effects. | |
dc.language.iso | englanti | |
dc.publisher | MDPI AG | |
dc.relation.ispartofseries | International journal of molecular sciences | |
dc.relation.uri | http://dx.doi.org/10.3390/ijms21062066 | |
dc.rights | CC BY 4.0 | |
dc.subject | hydroquinone | |
dc.subject | Resvega | |
dc.subject | retinal pigment epithelial cell | |
dc.subject | ROS | |
dc.subject | ARPE-19 | |
dc.subject | cell viability | |
dc.subject | inflammation | |
dc.subject | antioxidant | |
dc.subject | NF-κB | |
dc.subject | NADPH oxidase | |
dc.title | Resvega Alleviates Hydroquinone-Induced Oxidative Stress in ARPE-19 Cells | |
dc.description.version | published version | |
dc.contributor.department | School of Pharmacy, Activities | |
dc.contributor.department | School of Medicine / Clinical Medicine | |
uef.solecris.id | 69455678 | en |
dc.type.publication | Tieteelliset aikakauslehtiartikkelit | |
dc.relation.doi | 10.3390/ijms21062066 | |
dc.description.reviewstatus | peerReviewed | |
dc.publisher.country | Sveitsi | |
dc.relation.articlenumber | 2066 | |
dc.relation.issn | 1661-6596 | |
dc.relation.issue | 6 | |
dc.relation.volume | 21 | |
dc.rights.accesslevel | openAccess | |
dc.type.okm | A1 | |
uef.solecris.openaccess | Open access -julkaisukanavassa ilmestynyt julkaisu | |
dc.rights.copyright | © Authors | |
dc.type.displayType | article | en |
dc.type.displayType | artikkeli | fi |
dc.rights.url | https://creativecommons.org/licenses/by/4.0/ | |