Dataset Figure_6: Cln3 levels pulse prior to Start
Research data
Citation
Litsios, Athanasios (University of Groningen); Goswami, Pooja (Biological Sciences, Rensselaer Polytechnic Institute, Troy NY 12180, USA); Terpstra, Hanna M. (University of Groningen); Coffin, Carleton (Biological Sciences, Rensselaer Polytechnic Institute, Troy NY 12180, USA); Vuillemenot, Luc-Alban (University of Groningen); Rovetta, Mattia (University of Groningen); Ghazal, Ghada (Institute for Research in Immunology and Cancer, University of Montréal, Montréal, Quebec, Canada); Guerra, Paolo (University of Groningen); Buczak, Katarzyna (Proteomics Core Facility, Biozentrum, University of Basel, 4056 Basel, Switzerland); Schmidt, Alexander (Proteomics Core Facility, Biozentrum, University of Basel, 4056 Basel, Switzerland); Tollis, Sylvain (Institute for Research in Immunology and Cancer, University of Montréal, Montréal, Quebec, Canada and Institute of Biomedicine, University of Eastern Finland, FI-70210 Kuopio, Finland); Tyers, Mike (Institute for Research in Immunology and Cancer, University of Montréal, Montréal, Quebec, Canada); Royer, Catherine A. (Biological Sciences, Rensselaer Polytechnic Institute, Troy NY 12180, USA); Milias-Argeitis, Andreas (University of Groningen); Heinemann, Matthias (University of Groningen). , Dataset Figure_6: Cln3 levels pulse prior to Start, 2022, https://doi.org/10.34894/PKZZOJ.Licensed under
Abstract
Data and Matlab script
1. FLmean_Cln3_GLU: CSV file containing mean GFP concentration from Cln3-P2A-GFP for single daughter cells grown in glucose. Each cell is followed from birth until a few minutes after bud appearance. Data is organized by columns (one column corresponds to one cell). The first column is the vector of measurement time points (in minutes).
2. Vol_Cln3_GLU: CSV file containing cell volume for each of the daughter cells described above. Data is organized by columns (one column corresponds to one cell). The first column is the vector of measurement time points (in minutes).
3. bud_times: Excel file containing the time of bud appearance for each of the daughter cells described above
4. GP_FLtot_Cln3_GLU_example: Matlab script used to perform Gaussian process regression (see description in Methods) on the total GFP fluorescence for each of the daughter cells described above. The script produces single-cell Cln3 abundance data used to generate Fig. 5c of the main text and Extended Data Figure 4H.
NOTE: in order to run, the Matlab script requires the installation of the GPML Matlab toolbox (freely available at http://www.gaussianprocess.org/gpml/code/matlab/doc/).
