dc.contributor.author | Mustafa, Ehab | |
dc.contributor.author | Luukkonen, Jukka | |
dc.contributor.author | Makkonen, Jenny | |
dc.contributor.author | Naarala, Jonne | |
dc.date.accessioned | 2021-08-18T10:19:36Z | |
dc.date.available | 2021-08-18T10:19:36Z | |
dc.date.issued | 2021 | |
dc.identifier.uri | https://erepo.uef.fi/handle/123456789/25968 | |
dc.description.abstract | We investigated the effects of 50 Hz extremely low-frequency magnetic fields (MFs) on gene expression related to the circadian rhythm or DNA damage signaling and whether these fields modify DNA damage repair rate after bleomycin treatment. Murine FDC-P1 hematopoietic cells were exposed for different durations (15 min, 2 h, 12 h, and 24 h) to either 200 μT MFs or sham-exposures. Cells were then collected for comet assay or real-time PCR to determine immediate DNA damage level and circadian rhythm gene expression, respectively. To assess DNA-damage signaling and DNA repair rate, the cells were subsequently treated with 20 μg/mL bleomycin for 1 h and then either assayed immediately or allowed to repair their DNA for 1 or 2 h. We found that circadian rhythm-related genes were upregulated after 12 h of MF exposure and downregulated after 24 h of MF exposure, but none of the affected genes were core genes controlling the circadian rhythm. In addition, we found that the repair rate for bleomycin-induced damage was only decreased after MF exposure for 24 h. In conclusion, our findings suggest that the effects of MFs are duration-dependent; they were observed predominantly after long exposures. | |
dc.language.iso | englanti | |
dc.publisher | Elsevier BV | |
dc.relation.ispartofseries | Mutation research | |
dc.relation.uri | http://dx.doi.org/10.1016/j.mrfmmm.2021.111756 | |
dc.rights | CC BY 4.0 | |
dc.subject | magnetic fields | |
dc.subject | circadian rhythm | |
dc.subject | DNA damage | |
dc.subject | DNA repair | |
dc.subject | gene expression | |
dc.title | The duration of exposure to 50 Hz magnetic fields: Influence on circadian genes and DNA damage responses in murine hematopoietic FDC-P1 cells | |
dc.description.version | published version | |
dc.contributor.department | Ympäristö- ja biotieteiden laitos / Toiminta | |
uef.solecris.id | 80088769 | en |
dc.type.publication | Tieteelliset aikakauslehtiartikkelit | |
dc.relation.doi | 10.1016/j.mrfmmm.2021.111756 | |
dc.description.reviewstatus | peerReviewed | |
dc.publisher.country | Alankomaat | |
dc.relation.articlenumber | 111756 | |
dc.relation.issn | 0027-5107 | |
dc.relation.volume | 823 | |
dc.rights.accesslevel | openAccess | |
dc.type.okm | A1 | |
uef.solecris.openaccess | Hybridijulkaisukanavassa ilmestynyt avoin julkaisu | |
dc.rights.copyright | © 2021 The Authors | |
dc.type.displayType | article | en |
dc.type.displayType | artikkeli | fi |
dc.rights.url | https://creativecommons.org/licenses/by/4.0/ | |