dc.contributor.author | Sokka, Mikko | |
dc.contributor.author | Koalick, Dennis | |
dc.contributor.author | Hemmerich, Peter | |
dc.contributor.author | Syväoja, Juhani E | |
dc.contributor.author | Pospiech, Helmut | |
dc.date.accessioned | 2018-08-24T11:01:13Z | |
dc.date.available | 2018-08-24T11:01:13Z | |
dc.date.issued | 2018 | |
dc.identifier.uri | https://erepo.uef.fi/handle/123456789/6848 | |
dc.description.abstract | The mammalian DNA replication program is controlled at two phases, the licensing of potential origins of DNA replication in early gap 1 (G1), and the selective firing of a subset of licenced origins in the synthesis (S) phase. Upon entry into the S phase, serine/threonine-protein kinase ATR (ATR) is required for successful completion of the DNA replication program by limiting unnecessary dormant origin activation. Equally important is its activator, DNA topoisomerase 2-binding protein 1 (TopBP1), which is also required for the initiation of DNA replication after a rise in S-phase kinase levels. However, it is unknown how the ATR activation domain of TopBP1 affects DNA replication dynamics. Using human cells conditionally expressing a TopBP1 mutant deficient for ATR activation, we show that functional TopBP1 is required in suppressing local dormant origin activation. Our results demonstrate a regulatory role for TopBP1 in the local balancing of replication fork firing within the S phase. | |
dc.language.iso | englanti | |
dc.publisher | MDPI AG | |
dc.relation.ispartofseries | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | |
dc.relation.uri | http://dx.doi.org/10.3390/ijms19082376 | |
dc.rights | CC BY 4.0 | |
dc.subject | DNA replication | |
dc.subject | S phase | |
dc.subject | origin firing | |
dc.subject | TopBP1 | |
dc.subject | ATR | |
dc.title | The ATR-Activation Domain of TopBP1 Is Required for the Suppression of Origin Firing during the S Phase | |
dc.description.version | published version | |
dc.contributor.department | Ympäristö- ja biotieteiden laitos / Toiminta | |
dc.contributor.department | School of Medicine / Biomedicine | |
uef.solecris.id | 56657454 | en |
dc.type.publication | Tieteelliset aikakauslehtiartikkelit | |
dc.relation.doi | 10.3390/ijms19082376 | |
dc.description.reviewstatus | peerReviewed | |
dc.publisher.country | Sveitsi | |
dc.relation.articlenumber | 2376 | |
dc.relation.issn | 1661-6596 | |
dc.relation.issue | 8 | |
dc.relation.volume | 19 | |
dc.rights.accesslevel | openAccess | |
dc.type.okm | A1 | |
uef.solecris.openaccess | Open access -julkaisukanavassa ilmestynyt julkaisu | |
dc.rights.copyright | © Authors | |
dc.type.displayType | article | en |
dc.type.displayType | artikkeli | fi |
dc.rights.url | https://creativecommons.org/licenses/by/4.0/ | |