Asthmatic farm children show increased CD3+CD8low T-cells compared to non-asthmatic farm children
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CitationTwardziok M. Schröder PC. Krusche J. Casaca VI. Illi S. Böck A. Loss GJ. Kabesch M. Toncheva A. Roduit C. Depner M. Genuneit J. Renz H. Roponen M. Weber J. Braun-Fahrländer C. Riedler J. Lauener R. Vuitton DA. Dalphin JC et al.. the PASTURE Study Group. (2017). Asthmatic farm children show increased CD3+CD8low T-cells compared to non-asthmatic farm children. Clinical Immunology, 183, 285-292. 10.1016/j.clim.2017.09.009.
Several studies report an important role of CD8+ cytotoxic T-cells in atopy. Farm children show protection against atopy development, partly explained by CD4+ T-cell subtypes. Additional effects of CD8+ T-cells are unknown being investigated in this study within the PASTURE/EFRAIM birth cohort in PBMCs from farming and non-farming 6-year-old (N = 76) German children. CD3+ CD8+ CD25+ T-cells were analyzed by flow cytometry. Genotyping of 17q21 locus-SNPs associated with childhood asthma was performed. No differences in CD8+ T-cell subsets were seen between farmers and non-farmers regardless of asthma. Among farm children, asthmatics displayed increased CD3+ CD8low(CD25+) T-cells compared to non-asthmatics. Asthmatic farm children exhibited a lower PI-induced stimulatory capacity of CD3+ CD8low(CD25+) cells and a lower IFN-γ secretion than non-asthmatic farm children. Among farm children with GSDMB and ORMDL3 risk alleles, asthmatics displayed higher CD3+ CD8low cells than non-asthmatics. Our data indicates a specific role of CD8low T-cells in asthmatic farm children.