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dc.contributor.authorPoukka, M
dc.contributor.authorBykachev, A
dc.contributor.authorSiiskonen, H
dc.contributor.authorTyynelä-Korhonen, K
dc.contributor.authorAuvinen, P
dc.contributor.authorPasonen-Seppänen, S
dc.contributor.authorSironen, R
dc.date.accessioned2016-10-12T11:57:36Z
dc.date.available2016-10-12T11:57:36Z
dc.date.issued2016
dc.identifierhttp://doi.org/10.1186/s12885-016-2344-8fi_FI
dc.identifier.citationPoukka, M., Bykachev, A., Siiskonen, H., Tyynelä-Korhonen, K., Auvinen, P., Pasonen-Seppänen, S., & Sironen, R. (2016). Decreased expression of hyaluronan synthase 1 and 2 associates with poor prognosis in cutaneous melanoma. BMC Cancer, 16, 313. http://doi.org/10.1186/s12885-016-2344-8fi_FI
dc.identifier.issn1471-2407
dc.identifier.urihttps://erepo.uef.fi/handle/123456789/159
dc.descriptionArticle
dc.description.abstractBackground Hyaluronan is a large extracellular matrix molecule involved in several biological processes such as proliferation, migration and invasion. In many cancers, hyaluronan synthesis is altered, which implicates disease progression and metastatic potential. We have previously shown that synthesis of hyaluronan and expression of its synthases 1–2 (HAS1-2) decrease in cutaneous melanoma, compared to benign melanocytic lesions. Methods In the present study, we compared immunohistological staining results of HAS1 and HAS2 with clinical and histopathological parameters to investigate whether HAS1 or HAS2 has prognostic value in cutaneous melanoma. The specimens consisted of 129 tissue samples including superficial (Breslow ≤ 1 mm) and deep (Breslow > 4 mm) melanomas and lymph node metastases. The differences in immunostainings were analysed with non-parametric Mann–Whitney U test. Associations between immunohistological staining results and clinical parameters were determined with the χ2 test. Survival between patient groups was compared by the Kaplan-Meier method using log rank test and Cox’s regression model was used for multivariate analyses. Results The expression of HAS1 and HAS2 was decreased in deep melanomas and metastases compared to superficial melanomas. Decreased immunostaining of HAS2 in melanoma cells was significantly associated with several known unfavourable histopathologic prognostic markers like increased mitotic count, absence of tumor infiltrating lymphocytes and the nodular subtype. Furthermore, reduced HAS1 and HAS2 immunostaining in the melanoma cells was associated with increased recurrence of melanoma (p = 0.041 and p = 0.006, respectively) and shortened disease- specific survival (p = 0.013 and p = 0.001, respectively). Conclusions This study indicates that reduced expression of HAS1 and HAS2 is associated with melanoma progression and suggests that HAS1 and HAS2 have a prognostic significance in cutaneous melanoma.fi_FI
dc.language.isoENfi_FI
dc.publisherBioMed Central Ltd.fi_FI
dc.relation.ispartofseriesBMC CANCER
dc.relation.urihttp://doi.org/10.1186/s12885-016-2344-8fi_FI
dc.rightsCC BY http://creativecommons.org/licenses/by/4.0/fi_FI
dc.subjectHyaluronanfi_FI
dc.subjectMelanomafi_FI
dc.subjectHyaluronan synthases 1 and 2fi_FI
dc.subjectHyaluronidase 2fi_FI
dc.subjectPrognosisfi_FI
dc.subjectLymph node metastasisfi_FI
dc.titleDecreased expression of hyaluronan synthase 1 and 2 associates with poor prognosis in cutaneous melanomafi_FI
dc.typehttp://purl.org/eprint/type/JournalArticle
dc.description.versionPublisher's pdffi_FI
dc.contributor.departmentFaculty of Health Sciences
dc.contributor.departmentSchool of Medicine / Clinical Medicine
dc.contributor.departmentSchool of Medicine / Biomedicine
uef.solecris.id41377936
eprint.statushttp://purl.org/eprint/status/PeerReviewedfi_FI
dc.type.publicationinfo:eu-repo/semantics/article
dc.rights.accessrights© Authors
dc.relation.doi10.1186/s12885-016-2344-8
dc.description.reviewstatushttp://purl.org/eprint/status/PeerReviewed
dc.relation.articlenumber313
dc.relation.issn1471-2407
dc.relation.volume16


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