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Difference in the core-shell dynamics of polyethyleneimine and poly(L-lysine) DNA polyplexes

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Date
2017
Author(s)
Vuorimaa-Laukkanen E
Lisitsyna ES
Ketola T-M
Morin-Pickardat E
Liang H
Hanzlíková M
Yliperttula M
Unique identifier
10.1016/j.ejps.2017.03.025
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Citation
Vuorimaa-Laukkanen E. Lisitsyna ES. Ketola T-M. Morin-Pickardat E. Liang H. Hanzlíková M. Yliperttula M. (2017). Difference in the core-shell dynamics of polyethyleneimine and poly(L-lysine) DNA polyplexes.  EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 103, 122-127. 10.1016/j.ejps.2017.03.025.
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© Elsevier B.V
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CC BY-NC-ND https://creativecommons.org/licenses/by-nc-nd/4.0/
Abstract

Electrostatic polymer-DNA complexes (polyplexes) have been widely investigated for DNA delivery, and remarkable differences in transfection efficacy have been seen among the materials. For example, polyethyleneimine (PEI) mediates DNA transfection more effectively than poly(l-lysine) (PLL). Biophysical properties of the polyplexes may explain their different properties in gene delivery. We investigated the structural dynamics in DNA polyplexes, especially the material exchange between the core and shell regions of the PEI and PLL polyplexes. Steady-state fluorescence spectroscopy and double labeling based fluorescence resonance energy transfer (FRET) techniques were used to study the DNA polyplexes. According to our results there is a clear difference between these two polymers: core exchange takes place in PEI but not in PLL polyplexes. Such differences in structural dynamics of polyplexes explain, at least partly, the differences in DNA release and transfection efficacy at cellular level.

Subjects
Polyplex   DNA complexation   Fluorescence spectroscopy   Non-viral gene delivery   Polyethylene imine   Poly-l-lysine   
URI
https://erepo.uef.fi/handle/123456789/4254
Link to the original item
http://dx.doi.org/10.1016/j.ejps.2017.03.025
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