Long Non-Coding RNA Modulation of VEGF-A during Hypoxia
Self archived versionpublished version
MetadataShow full item record
CitationNieminen, T. Scott, TA. Lin, FM. Chen, Z. Yla-Herttuala, S. Morris, KV. (2018). Long Non-Coding RNA Modulation of VEGF-A during Hypoxia. Non-Coding RNA, 4 (4) , 34. 10.3390/ncrna4040034.
The role and function of long non-coding RNAs (lncRNAs) in modulating gene expression is becoming apparent. Vascular endothelial growth factor A (VEGF-A) is a key regulator of blood vessel formation and maintenance making it a promising therapeutic target for activation in ischemic diseases. In this study, we uncover a functional role for two antisense VEGF-A lncRNAs, RP1-261G23.7 and EST AV731492, in transcriptional regulation of VEGF-A during hypoxia. We find here that both lncRNAs are polyadenylated, concordantly upregulated with VEGF-A, localize to the VEGF-A promoter and upstream elements in a hypoxia dependent manner either as a single-stranded RNA or DNA bound RNA, and are associated with enhancer marks H3K27ac and H3K9ac. Collectively, these data suggest that VEGF-A antisense lncRNAs, RP1-261G23.7 and EST AV731492, function as VEGF-A promoter enhancer-like elements, possibly by acting as a local scaffolding for proteins and also small RNAs to tether.
Subjectsvascular endothelial growth factor A (VEGF-A) long non-coding RNA (lncRNA) transcriptional activation antisense RNA
Link to the original itemhttp://dx.doi.org/10.3390/ncrna4040034
- Terveystieteiden tiedekunta